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KMID : 0380020130280050315
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 5 p.315 ~ p.318
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Bioethanol Production from Eucheuma spinosum using Various Yeasts
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Kim Min-Ji
Kim Jung-Soo
Ra Chae-Hun
Kim Sung-Koo
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Abstract
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Ethanol fermentations were performed using separate hydrolysis and fermentation (SHF) processes with monosaccharides from pretreated seaweed, Eucheuma spinosum as the biomass. The pretreatment was carried out with 11% (w/v) seaweed slurry and 150 mM H2SO4 at 121oC for 40 min. Enzyme hydrolysis after H2SO4 pretreatment was performed with Celluclast 1.5 L at 45oC for 24 h. Five % active charcoal were added to hydrolysate to removed 5-hydroxy methylfurfural. Ethanol fermentation with 11% (w/v) seaweed hydrolysate was performed for 72~96 h using Kluyvermyces marxianus, Pichia stipits, Saccharomyces cervisiae and Candida tropicalis. Ethanol concentration was reached to 18 g/L by K. marxianus, 16 g/L by P. stipitis, 15 g/L by S. cerevisiae and 10 g/L by C. tropicalis, respectively. The ethanol yield from total monosugar was obtained 0.50 and ethanol productivity was obtained 0.38 g/L/h by K. marxianus.
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KEYWORD
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Seaweed, Eucheuma spinosum, Ethanol fermentation, Kluyveromyces marxinaus
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